Worcester HIV Vaccine

Publications

  1. Wang S, Chan KW, Wei D, Ma X, Liu S, Hu G, Park S, Pan R, Gu Y, Nazzari AF, Olia AS, Xu K, Lin BC, Louder MK, McKee K, Doria-Rose NA, Montefiori D, Seaman MS, Zhou T, Kwong PD, Arthos J, Kong XP, Lu S. Human CD4-binding site antibody elicited by polyvalent DNA prime-protein boost vaccine neutralizes cross-clade tier-2-HIV strains. 2024, Nat Commun, 15(1):4301. PMCID: PMC11109196. DOI: 10.1038/s41467-024-48514-8
  2. Frank, I., Li, S.S., Grunenberg, N., Overton, E.T., Robinson, S.T., Zheng, H., Seaton, K.E., Heptinstall, J.R., Allen, M.A., Mayer, K.H., Culver, D.A., Keefer, M.C., Edupuganti, S., Pensiero, M.N., Mehra, V.L., De Rosa, S.C., Morris, D.E., Wang, S., Seaman, M.S., Montefiori, D.C., Ferrari, G., Tomaras, G.D., Kublin, J.G.,  Corey, L., Lu, S. and the HVTN 124 Study Team. Safety and immunogenicity of a polyvalent DNA-protein HIV vaccine with matched Env immunogens delivered as a prime-boost regimen or coadministered in HIV-uninfected adults in the USA (HVTN 124): a phase 1, placebo-controlled, double-blind randomised controlled trial. 2024. The Lancet HIV. 11(5):e285-e299. PMID: 38692824. DOI: 10.1016/S2352-3018(24)00036-5.
  3. Wang S, Yates N, Pollara J, Voronin Y, Stanfield-Oakley S, Han D, Hu G, Li W, Ferrari G, Tomaras G, Lu S.  Broadly Binding and Functional 1 Antibody and Persisting Memory B cells elicited by HIV Vaccine PDPHV. Nature Group Journal/Vaccines. Feb 09, 2022, PMID: 35140230 DOI: 10.1038/s41541-022-00441-9
  4. Hioe CE, Li G, Liu X, Tsahouridis O, He X, Funaki M, Klingler J, Tang AF, Feyznezhad R, Heindel DW, Wang XH, Spencer DA, Hu G, Satija N, Prévost J, Finzi A, Hessell AJ, Wang S, Lu S, Chen BK, Zolla-Pazner S, Upadhyay C, Alvarez R, Su L. Non-neutralizing antibodies targeting the immunogenic regions of HIV-1 envelope reduce mucosal infection and virus burden in humanized mice. PLoS Pathog. 2022 Jan 5;18(1):e1010183. doi: 10.1371/journal.ppat.1010183. Epub ahead of print. PMID: 34986207.
  5. Li, Y., Wang, S., Hu, G., Zhang, L., Liu, S. and Lu, S. DNA priming immunization is more effective than recombinant protein vaccine in eliciting antigen-specific B cell responses. 2021. Emerging Microbes & Infections, 10:1, 833-841, DOI:10.1080/22221751.2021.1918026. PMID: 33853515
  6. Wang, S., Voronin, Y., Zhao P., Ishihara M., Mehta N., Porterfield M., Chen Y., Bartley C., Hu G., Han D., Wells L., Tiemeyer M., Lu S. Glycan profiles of gp120 protein vaccines from four major HIV-1 subtypes produced from different host cell lines under non-GMP or GMP conditions. 2020. J Virol, Jan 15;  doi: 10.1128/JVI.01968-19 , PMID: 31941770.
  7. Quitadamo, B., Peters, P., Repik, A., O’Connell, O., Mou, Z., Koch, M., Somasundaran, M., Brody, R., Luzuriaga, K., Wallace, A., Wang, S., Lu, S., McCauley, S., Luban, J., Duenas-Decamp, M., Gonzalez-Perez, M. and Clapham, P. HIV-1 R5 macrophage-tropic envelope glycoprotein trimers bind CD4 with high affinity, while the CD4 binding site on non-macrophage-tropic, T-tropic R5 envelopes is occluded. 2018. J Virol, Jan 2;92(2). doi: 10.1128/JVI.00841-17. PMID: 29118121.
  8. Chan, K-W., Pan, R., Costa, M., Gorny, M., Wang, S., Lu, S. and Kong, X-P. Structural comparison of human anti-HIV-1 gp120 V3 mAbs of the same gene usage induced by vaccination and chronic infection. 2018. J Virology, July 11. doi: 10.1128JVI.00641-18. PMID: 29997214.
  9. Flotte, T. and Lu, S. DNA Vaccination in 2018: An Update. 2018. Hum Gene Ther, 29(9):963–965. doi: 10.1089/hum.2018.29073.trf. PMID: 30230936.
  10. Lertjuthaporn, S., Cicala, C., Van Ryk, D., Liu, M., Yolitz, J., Wei, D., Nawaz, F., Doyle, A., Park, C., Lu, S., Lou, Y., Wang, S., Pan, R., Jiang, X., Villinger, F., Byrareddy, S., Santangelo, P., Morris, L., Wibmer, C., Mason, R., Gorman, J., Hiatt, J., Martinelli, E., Roederer, M., Gorini, G., Franchini, G., Fauci, A., Ansari, A., Kong, X-P., Pattanapanyasat, K. and Arthos, J. Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to a4b7. 2018. PLoS Pathog, 14(8): e1007278. doi: 10.1371/journal.ppat.1007278. PMID: 30153309.
  11. Balasubramanian, P., Kumar, R., Williams, C., Itri, V., Wang, S., Lu, S., Hessell, A.J., Haigwood, N.L., Sinangil, F., Higgins, K., Liu, L., Gorny, M.K., Totrov, M., Nadas, A., Kong, X.-P., Zolla-Pazner, S., and Hioe, C. E. Differential induction of anti-V3 crown antibodies with cradle- and ladle-binding modes in response to HIV-1 envelope vaccination. 2017. Vaccine, 35:1464-1473. PMID: 28185743.
  12. Li, X., Grant, O., Ito, K., Wallace, A., Wang, S., Zhao, P., Wells, L., Lu, S., Woods, R., Sharp, J. Structural analysis of the glycosylated intact HIV‑1 gp120−b12 antibody complex using hydroxyl radical protein footprinting. 2017. Biochemistry, 56(7): 957–970. PMID: 28102671.
  13. Farfán-Arribas, D., Liu, S., Wang, S. and Lu, S. The dynamics of immunoglobulin V-gene usage and clonotype expansion in mice after prime and boost immunizations as analyzed by NGS. 2017. Hum Vaccin Immunother, Dec 2;13(12):2987-2995. doi: 10.1080/21645515.2017.1379638. PMID: 29049006.
  14. Wang, S., Wu, T-H., Hackett, A., Efros, V., Wang, Y., Han, D., Wallace, A., Chen, Y., Hu, G., Liu, S., Clapham, P., Arthos, J., Montefiori, D. and Lu, S. Screening of primary gp120 immunogens to formulate the next generation polyvalent DNA prime-protein boost HIV-1 vaccines. 2017. Hum Vaccin Immunother, Dec 2;13(12):2996-3009. doi: 10.1080/21645515.2017.1380137. PMID: 28933684.
  15. Marty-Roix, R., Vladimer, G., Pouliot, K., Weng, D., Buglione-Corbett, R., West, K., MacMincking, J., Chee, J., Wang, S., Lu, S., Lien, E. Identification of QS-21 as an inflammasome-activation molecular component of saponin adjuvants. 2016. J Biol Chem, 15; 291(3): 1123-36. PMID: 26555265.
  16. Wu, H., Chen, Y., Liu, H., Lin-Lin, X., Teuscher, P., Wang, S., Lu, S., Dent, A. Follicular regulatory T cells repress cytokine production by follicular helper T cells and optimize IgG responses in mice. 2016. Eur J Immunol, 00:1-10. PMID: 26887860.
  17. Zolla-Pazner, S., Powell, R., Yahyaei, S., Williams, C., Jiang, X., Li, W., Lu, S., Wang, S., Upadhyay, C., Hioe, C., Totrov, M., and Kong, X.-P. Rationally-designed vaccines targeting the V2 region of HIV-1 gp120 induce a focused, cross clade-reactive, biologically functional antibody response. 2016. J Virology. pii: JVI.01403-16. PMID: 27630234.
  18. Costa, M., Pollara, J., Edwards, R.W., Seaman, M., Gorny, M.K., Montefiori, D., Liao, H.-X., Ferrari, G., Lu, S., and Wang, S. Fc receptor mediated activities by Env-specific human monoclonal antibodies generated from volunteers receiving a DNA prime-protein boost HIV vaccine DP6-001. 2016. J Virology. 90(22):10362-10378. PMID: 27630232.
  19. Jiang, X., Totrov, M., Li, W., Sampson, J., Williams, C., Lu, H., Wu, X., Lu, S., Wang, S., Zolla-Pazner, S., and Kong, X.-P. Rationally designed immunogens targeting HIV-1 gp120 V1V2 induce distinct conformation-specific antibody responses in rabbits. 2016.J Virol, pii: JVI.01409-16.PMID: 27707920.
  20. Pan, R., Chen, Y., Vaine, M., Hu, G., Wang, S., Lu, S., Kong, X.-P. Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding. 2015. Emerg Microbes Infect, 4, e44; doi:10.1038/emi.2015.44. PMID: 26251831.
  21. Buglione-Corbett, R., Pouliot, K., Marty-Roix, R., Li, W., West, K., Wang, S., Baz Morelli, A., Lien, E., Lu, S. Reduced MyD88 dependency of ISCOMATRIX™ adjuvant in a DNA prime-protein boost HIV vaccine. 2014. Hum Vaccin Immunother, 10(4): 1-13. PMID: 24513632.
  22. Chen, Y., Wang, S., Lu, S. DNA immunization for HIV vaccine development. 2014. Vaccines, 2(1): 138-159.doi: 10.3390/vaccines2010138. PMID: 26344472.
  23. Hollister, K., Chen, Y., Wang, S., Wu, H., Mondal, A., Clegg, N., Lu, S., Dent, A. The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination. 2014. Hum Vaccin Immunother, 10:7, 1985–1992. PMID: 25424808.
  24. Pouliot, K., Buglione-Corbett, R., Marty-Roix, R., Montminy-Paquette, S., West, K., Wang, S., Lu, S., Lien, E.Contribution of TLR4 and MyD88 for adjuvant monophosphoryl lipid A (MPLA) activity in a DNA prime–protein boost HIV-1 vaccine. 2014. Vaccine, 32: 5049–5056. PMID: 25045815.
  25. Lu, S. Editorial overview: Vaccines against challenging viral pathogens and new vaccine technology. 2014. Curr Opin Virol, 6: viii-ix. PMID: 24831092.
  26. Lu, S. Novel vaccine technology and the future of vaccine science. 2014. Hum Vaccin Immunother, 10:5, 1143-1146, DOI: 10.4161/hv.29042. PMID: 25290657.
  27. O’Connell, O., Repik, A., Reeves, J.D., Gonzalez-Perez, M.P., Quitadamo, B., Anton, E.D., Duenas-Decamp, M., Peters, P., Lin, R., Zolla-Pazner, S., Corti, D., Wallace, A., Wang, S., Kong, X-P., Lu, S., Clapham, P. Efficiency of bridging-sheet recruitment explains HIV-1 R5 Envelope glycoprotein sensitivity to soluble CD4 and macrophage tropism. 2013. J Virology, 87(1):187-198. PMID: 23055568.
  28. Wallace, A., West, K., Rothman, A., Ennis, F.A., Lu, S., Wang, S. Post-translational intracellular trafficking determines the type of immune response elicited by DNA vaccines expressing Gag antigen of human immunodeficiency virus type 1 (HIV-1). 2013. Hum Vaccin Immunother, 9(10):2095-102. PMID: 23941868.
  29. Buglione-Corbett, R., Pouliot, K., Marty-Roix, R., West, K., Wang, S., Lien, E., Lu, S. Serum cytokine profiles associated with specific adjuvants used in a DNA prime-protein boost vaccination strategy. 2013. PLoS One, 8(9), e74820. PMID: 24019983.
  30. Chen, Y., Vaine, M., Wallace, A., Han, D., Wan, S., Seaman, M.S., Montefiori, D., Wang, S., Lu, S.A novel rabbit monoclonal antibody platform to dissect the diverse repertoire of antibody epitopes for HIV-1 Env immunogen design. 2013. J Virology, 87(18): 10232-10243. PMID: 23864612.
  31. Pan, R., Sampson, J.M., Chen, Y., Vaine, M., Wang, S., Lu, S., Kong, X-P. Rabbit anti-HIV-1 monoclonal antibodies raised by immunization can mimic the antigen-binding modes of antibodies derived from HIV-1 infected humans. 2013. J Virology, 87(18): 10221-10231. PMID: 23864637.
  32. Montero, M., Gulzar, K-A., Donald, J.E., Lepik, C., Wu, S., Tsai, S., Julien, J-P., Hessell, A.J., Wang, S., Lu, S., Burton, D.R., Pai, E.F., DeGrado, W.F., Scott, J.K. Neutralizing epitopes in the membrane-proximal external region of HIV-1 gp41 are influenced by the transmembrane domain and the plasma membrane. 2012. J Virology, 86(6):2930. PMID: 22238313.
  33. Zhang, M., Jiao, Y., Wang, S., Zhang, L., Huang, Z., Chen, Y., Wu, H., Lu, S. Serum neutralizing activities from a Beijing homosexual male cohort infected with different subtypes of HIV-1in China. 2012. PLoS One, 7(10): e47548. PMID: 23094060
  34. Zhang, M., Zhang, L., Zhang, C., Hong, K., Shao, Y., Huang, Z., Wang, S., Lu, S. DNA prime-protein boost using subtype consensus Env was effective in eliciting neutralizing antibody responses against subtype BC HIV-1 viruses circulating in China. 2012. Hum Vaccin Immunother, 8(11):1630-1637. PMID: 23111170.
  35. Wang, S., Kishko, M., Wan, S., Wang, Y., Brewster, F., Gray, G.E, Violari, A., Sullivan, J.L., Somasundaran, M., Luzuriaga,K., Lu, S. Pilot study on the immunogenicity of paired Env immunogens from mother-to-child transmitted HIV-1 isolates. 2012. Hum Vaccin Immunother, 8(11): 1638-1647. PMID: 23151449
  36. Hellerstein, M., Xu, Y., Marino, T., Lu, S., Yi, H., Wright, E.R., Robinson, H.L. Co-expression of HIV-1 virus-like particles and granulocyte-macrophage colony stimulating factor by GEO-D03 DNA vaccine. 2012. Hum Vaccin Immunother, 8(11):1654-1658 PMID: 23111169.
  37. Nie, J, Wang W, Wen, Z, Song, A, Hong, K, Lu, S, Zhong, P., Xu, J, Kong, W, Li, J, Shang, H, Ling, H, Ruan, L, Wang, Y. Optimization and proficiency testing of a pseudovirus-based assay for detection of HIV-1 neutralizing antibody in China. 2012. J Virol Methods, 185: 267–275. PMID: 22796285.
  38. Wang, Z., Zhang, M., Wang, Y., Jiao, Y., Zhang, L., Li, L., Huang, Z., Wu, H., Li, J., Lu, S., Wang, S. A versatile vector for the production of pseudotyped viruses expressing gp120 antigens from different clades of primary HIV-1 isolates. 2011. J Virol Methods, 171 (1): 183-189. PMID: 21034776.
  39. Vaine, M., Duenas-Decamp, M., Peters, P., Liu, Q., Arthos, J., Wang, S., Clapham, P., Lu, S. Two closely related Env antigens from the same patient elicited different spectra of neutralizing antibodies against heterologous HIV-1 isolates. 2011. J Virology, 85 (10): 4927-4936. PMID: 21411542.
  40. Zolla-Pazner, S., Kong, X-P., Jiang, X., Cardozo, T., Nadas, A., Cohen, S., Totrov, M., Seaman, M.S, Wang, S., Lu, S. Cross-clade HIV-1 neutralizing antibodies induced with V3-scaffold protein immunogens following priming with gp120 DNA. 2011. J Virology, 85(19):9887-9898. PMID: 21795338.
  41. Lu, S. (Commentary) Two is better than one. 2011. Lancet Inf Dis, 11(12): 889-891. PMID: 21975267.
  42. Clapham, P. and Lu, S. Precisely tuned antibodies nab HIV. 2011. Nature, News and Views, 477:416-417. PMID: 21938063.
  43. Abdel-Motal, U.M., Wang, S., Awad, A., Lu, S., Wigglesworth, K., Galili, U. Increased immunogenicity of HIV-1 p24 and gp120 following immunization with gp120/p24 fusion protein vaccine expressing alpha-Gal epitopes. 2010. Vaccine, 28:1758-1765. PMID: 20034607.
  44. Vaine, M., Wang, S., Hackett, A., Arthos, J., Lu, S. Antibody responses elicited through homologous or heterologous prime-boost DNA and protein vaccinations differ in functional activity and avidity. 2010. Vaccine, 28:2999-3007. PMID: 20170767.
  45. Jiao, Y., Zhang, T., Wang, S., Liu, Q., Zhou, H., Lu, S. and Wu, H. Profile of HIV-1 infected patients from an AIDS clinic in Beijing from 2007-2008. 2010. Curr HIV Res, 8(7): 515-520. PMID: 21073440.
  46. Vaine, M., Wang, S., Liu, Q., Arthos, J., Montefiori, D., Goepfert, P., McElrath, J.M., Lu, S. Profiles of human serum antibody responses elicited by three leading HIV vaccines focusing on the induction of Env-specific antibodies. 2010. PLoS ONE, 5(11):e13916. PMID: 21085486.
  47. Totrov, M., Jiang, X., Kong, X-P., Cohen, S., Krachmarov, C., Salomon, A., Williams, C., Seaman, M.S., Cardozo, T., Gorny, M.K., Wang, S., Lu, S., Pinter, A., Zolla-Pazner, S. Structure-guided design and immunological characterization of immunogens presenting the HIV-1 gp120 V3 loop on a CTB scaffold. 2010. Virology, 405:513-523. PMID: 20663531.
  48. Lu, S., Grimes-Serrano, J.M., Wang, S. Polyvalent AIDS vaccines (review). 2010. Curr HIV Res, 8(8): 622-629. PMID: 21054250.
  49. Cardozo, T., Kong, X., Totrov, M., Wang, S., Lu, S., Gorny, M., Pinter, A., Seaman, M. and Zolla-Pazner, S. 2010. Engineered immunogen presenting an epitope recognized by a neutralizing mAb elicits mammalian serum that recapitulate the mAb’s specificity. AIDS research & human retroviruses, 26(10):A26-A27
  50. Zolla-Pazner, S., Cohen, S., Pinter, A., Krachmarov, C., Wrin, T., Wang, S., Lu, S. Cross-clade neutralizing antibodies against HIV-1 induced in rabbits by focusing the immune response on a neutralizing epitope. 2009. Virology, 392(1):82-93. PMID: 19632700.
  51. Wang, Z., Wang S., Liu, S., Bao, Z., Zhuang, D., Li, L., Zhang, C., Zhang, L, Li, J. and Lu, S. Immunogenicities of Env glycoproteins from circulating HIV-1 isolates in China focusing on the strategy of DNA prime plus protein boost. 2009. Chinese Medical Journal (English edition), 122(19):2339-2345. PMID: 20079137.
  52. Vaine, M., Lu, S., Wang, S. Progress on the induction of neutralizing antibodies against HIV type 1 (HIV-1). 2009. BioDrugs 23(3):137-153. PMID: 19627166.
  53. Lu, S. Heterologous prime-boost vaccination. 2009. Curr Opin Immunol, 21:346-51. PMID: 19500964.
  54. Lu, S. (Commentary). Gene-based adjuvants: A new meaning. 2009. Hum Gene Ther, 20(10):1101-1102. PMID: 19848597.
  55. Zolla-Pazner, S., Sharpe-Cohen, S., Krachmarov, C., Wang, S., Pinter, A., Lu, S. Focusing the immune response on the V3 loop, a neutralizing epitope of the HIV-1 gp120 envelope. 2008. Virology, 372:233-246. PMID: 18061228.
  56. Wang, S., Kennedy, J.S., West, K., Montefiori, D.C., Coley, S., Lawrence, J., Shen, S., Green, S., Rothman, A.L., Ennis, F.A., Arthos, J., Pal, R., Markham, P., Lu, S. Cross-subtype antibody and cellular immune responses induced by a polyvalent DNA prime-protein boost HIV-1 vaccine in healthy human volunteers. 2008. Vaccine, 26(8):1098-1110. PMID: 18243434
  57. Bansal, A., Jackson, B., West, K., Wang, S., Lu, S., Kennedy, J.S., Goepfert, P.A. Multifunctional T-cell characteristics induced by a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 vaccine regimen given to healthy adults are dependent on the route and dose of administration. 2008. J Virology, 82(13):6458-6469. PMID: 18448544.
  58. Kennedy, J.S., Co., M., Green, S., Longtine, K., Longtine, J., O’Neil, M.A., Adams, J.P., Rothman, A.L., Yu, Q., Johnson-Leva, R., Pal, R., Wang, S., Lu, S., Markham, P. The safety and tolerability of an HIV-1 DNA prime-protein boost vaccine (DP6-001) in healthy adult volunteers. 2008. Vaccine, 26:4420-4424. PMID: 18588934.
  59. Vaine, M., Wang, S., Crooks, E.T., Jiang, P., Montefiori, D.C., Binley, J., Lu, S. Improved induction of antibodies against key neutralizing epitopes by human immunodeficiency virus type 1 gp120 DNA prime-protein boost vaccination compared to gp120 protein-only vaccination. 2008. J Virology, 82(15):7369-7378. PMID: 18495775.
  60. Lu, S., Wang, S., Grimes-Serrano, J.M. Current progress of DNA vaccine studies in humans. Expert Rev Vaccines, 2008, 7(2):175-191. PMID: 18324888.
  61. Lu, S. (Editorial) Human versus HIV: round 2 defeat in AIDS vaccine development. Expert Rev Vaccines, 2008, 7(2):151-153. PMID: 18324884.
  62. Lu, S. (Commentary) Immunogenicity of DNA vaccines in humans: It takes two to tango. 2008, Hum Vaccin, 4(6): 449-452. PMID: 18443427.
  63. Pal, R., Yu, Q., Wang, S., Kalyanaraman, V.S., Nair, B.C., Hudacik, L., Whitney, S., Keen, T., Hung, C-L., Hocker, L., Kennedy, J., Markham, P., Lu, S. Definitive toxicology and biodistribution study of a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 (HIV-1) vaccine in rabbits. 2006. Vaccine, 24(8):1225-34. PMID: 16219399.
  64. Cristillo, A.D., Wang, S., Caskey, M.S., Unangst, T., Hocker, L., He, L., Hudacik, L., Whitney, S., Keen, T., Chou., T-H.W., Shen, S., Joshi, S., Kalyanaraman, V.S., Nair, B.C., Markham, P., Lu, S., Pal, R. Preclinical evaluation of cellular immune responses elicited by a polyvalent DNA prime/protein boost HIV-1 vaccine. 2006. Virology, 346:151-168. PMID: 16325880.
  65. Wang, S., Farfan-Arribas, D.J., Shen, S., Chou, T-H.W., Hirsch, A., He, F., Lu, S. Relative contributions of codon usage, promoter efficiency and leader sequence to the antigen expression and immunogenicity of HIV-1 Env DNA vaccine. 2006. Vaccine, 24 (21):4531-4540. PMID: 16140431.
  66. Pal, R., Wang, S., Kalyanaraman, V.S., Nair, B.C., Whitney, S., Keen, T., Hocker, L., Hudacik, L., Rose, N., Mboudjeka, I., Shen, S., Chou, T.H.W., Montefiori, D., Mascola, J., Markham, P., Lu, S. Immunization of rhesus macaques with a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 vaccine elicits protective antibody response against simian human immunodeficiency virus of R5 phenotype.2006. Virology, 348:341-353. PMID: 16460776.
  67. Wang, S., Pal, R., Mascola, J.R., Chou, T-H.W., Mboudjeka, I., Shen, S., Liu, Q., Whitney, S., Keen, T., Nair, B.C., Kalyanaraman, V.S., Markham, P., Lu, S. Polyvalent HIV-1 Env vaccine formulations delivered by the DNA priming plus protein boosting approach are effective in generating neutralizing antibodies against primary human immunodeficiency virus type 1 isolates from subtypes A, B, C, D and E. 2006. Virology, 350:34-47. PMID: 16616287.
  68. Abdel-Motal, U., Wang, S., Lu, S., Wigglesworth, K., Galili, U. Increased immunogenicity of human immunodeficiency virus gp120 engineered to express Gal{alpha}1-3Gal{beta}1-4GlcNAc-R epitopes. 2006. J Virology, 80(14):6943-51. PMID: 16809300.
  69. Lu, S. Combination DNA plus protein HIV vaccines. 2006. Springer Seminar in Immunopathology, 28:255-265. PMID: 17021720.
  70. Wang, S., Arthos, J., Lawrence, J.M., Van Ryk, D., Mboudjeka, I., Shen, S., Chou, T-H.W., Montefiori, D.C., Lu, S. Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1. 2005. J Virology, 79(12):7933-7937. PMID: 15919951.
  71. Pal, R., Wang, S., Kalyanaraman, V.S., Nair, B.C., Whitney, S., Keen, T., Hocker, L., Hudacik, L., Rose, N., Cristillo, A., Mboudjeka, I., Shen, S., Chou, T-H.W., Montefiori, D., Mascola, J., Lu, S., Markham, P. Polyvalent DNA prime and envelope protein boost HIV-1 vaccine elicits humoral and cellular responses and controls plasma viremia in rhesus macaques following rectal challenge with an R5 SHIV isolate. 2005. J Med Primatology, 34(5-6):226-236. PMID: 16128917.
  72. Hurwitz, J., Slobod, K., Lockey, T.,Wang, S., Chou, T-H., and Lu, S. Application of a polyvalent approach to HIV vaccine development. 2005.Curr Drug Targets Infect Disord, 5(2):143-156. PMID: 15975020.
  73. Liu,F., Mboudjeka, I., Shen, S., Chou, T-H.W., Wang, S., Ross, T.M., Lu, S. Independent but not synergistic enhancement to the immunogenicity of DNA vaccine expressing HIV-1 gp120 glycoprotein by codon optimization and C3d fusion in a mouse model. 2004. Vaccine, 22:1764-1772. PMID: 15068860.
  74. Lu, S., Nucleic Acid Vaccines. 2004. J Nanjing Medical University, 18(5):221-224.
  75. Rasmussen, R.A., Hofmann-Lehmann, R., Montefiori, D.C., Li, P-L, Vlasak, J., Baba, T.W., Schmitz, J.E., Kuroda, M.J., Robinson, H.L., McClure, H.M., Lu, S., Hu, S-L, Rizvi, T.A. and Ruprecht, R.M. DNA prime/protein boost vaccination and sequential simian human immunodeficiency virus challenges in infant macaques: no original antigenic sin. 2002. J Med Primatology, 31:40-60. PMID: 12076047.
  76. Barnett, S.W., Lu, S., Srivastava, I., Cherpelis, S., Gettie, A., Blanchard, J., Wang, S., Mboudjeka, I., Leung, L., Lian, Y., Fong, A., Buckner, C., Ly, A., Hilt, S., Ulmer, J., Wild, C.T., Mascola, J.R., Stamatatos, L. The ability of an oligomeric human immunodeficiency virus type 1 (HIV-1) envelope antigen to elicit neutralizing antibodies against primary HIV-1 isolates is improved following partial deletion of the second hypervariable region. 2001. J Virology, 75(12): 5526-5540. PMID: 11356960.
  77. Robinson, H.L., Montefiori, D.C., Johnson, R.P., Manson, K.H., Kalish, M.L., Lifson, J.D., Rizvi, T.A., Lu, S., Hu, S-L., Mazzara, G.P., Panicalli, D.L., Herndon, J.G., Glickman, R., Candido, M.A., Lydy, S.L., Wyand, M.S., McClure, H.M. Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations. 1999. Nat Med, 5(5):526-534. PMID: 10229229.
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